1. China Three Gorges University Alan G. MacDiarmid Institute for Renewable Energy, Yichang 443002, China 2. College of Mechanical and Material Engineering, China Three Gorges University, Yichang 443002, China
Abstract:The reactions were studied for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent with fluorescence spectrophotometry. In the B-R medium, o-HPF reacts with BSA to form the stable complex compound. The reaction system of o-HPF and BSA was binary system, and the method for the determination of BSA was of good selectivity and stability and simplicity. The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 μg·mL-1 The quenching constant and thermodynamics constant of the fluorescence effects between BSA and o-HPF were calculated. The results showed that the non-covalent binding forces were the binding force between o-HPF and BSA.
Key words:o-hydroxyphenylfluorone (o-HPF);Bovine Serum Albumin (BSA);Fluorescence spectrophotometry
黄应平1,顾彦2,方艳芬1,刘玉良1,朱圣姬1,罗光富1. 邻羟基苯基荧光酮荧光光度法测定牛血清白蛋白[J]. 光谱学与光谱分析, 2008, 28(06): 1336-1339.
HUANG Ying-ping1,GU Yan2,FANG Yan-fen1,LIU Yu-liang1,ZHU Sheng-ji1,LOU Guang-fu1. Study on the Determination of Bovine Serum Albumin Using o-Hydroxyphenylfluorone Reagent with Fluorescence Spectrophotometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1336-1339.
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